Policy document
July 26th 2013

Test No. 236: Fish Embryo Acute Toxicity (FET) Test

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OECD
OECD
https://www.oecd.org Flag of igo IGO

The Organisation for Economic Co-operation and Development is an intergovernmental organization with 38 member countries, founded in 1961 to stimulate economic progress and world trade. It is a forum the members of which are countries describing themselves as committed to democracy and the market economy, providing a platform to compare policy experiences, seek answers to common problems, identify good practices and coordinate domestic and international policies of its members. The majority of OECD members are high-income economies with a very high Human Development Index (HDI) and are regarded as developed countries.
The test method described in this Test Guidelineis inteneded to determine the acute or letal toxicity of chemicals on embryonic stages of fish (Danio rerio).Newly fertilised zebrafish eggs are exposed to the test chemical for a period of 96 hrs. Every 24 hrs. Twenty embryos (one embryo per well) are exposed to the chemical tested at each concentration level. The test includes five increasing concentrations of the chemical tested and a control. Every 24 hours, four apical observations are recorded as indicators of lethality: (i) coagulation of fertilised eggs, (ii) lack of somite formation, (iii) lack of detachment of the tail-bud from the yolk sac, and (iv) lack of heartbeat. At the end of the exposure period, acute toxicity is determined based on a positive outcome in any of the four apical observations recorded, and the LC50 is calculated. The test report also includes a number of other important information elements related to the conduct of the test, in particular: the concentration of dissolved o...

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Identifiers

Overton ID
oecddiscovery-b2ee5ad05cab9ce6113ad1688963f596
doi
10.1787/9789264203709-en

Topics in this document

Calcium carbonate PH Zebrafish Physical sciences Chemistry Hard water Solution (chemistry) Spawn (biology) Water Sodium hydroxide Somite Solubility Chemical substance Magnesium

Related COFOG Divisions

5: Environmental Protection 5.3: Pollution Abatement 7: Health 7.5: R&D Health

Cites research funded by

OpenAlex NIH DOIs (April 2024) 1 Promotion and Mutual Aid Corporation for Private Schools of Japan 1 Japan Society for the Promotion of Science (JSPS 1 Grant-in-Aid for JSPS Fellows 1 Akiyama Foundation 1 Ministry of Education 1 National Institute of Health 1 Superfund Research Program 1 National Institutes of Health 1 AIAA Foundation 1 Japan Society for the Promotion of Science 1 U.S. Department of Health and Human Services 1 Ministry of Education, Culture, Sports, Science and Technology 1

Citations

Cited by 77 other policy documents (57 of them are from other policy sources)