Policy document
July 28th 2015

Test No. 241: The Larval Amphibian Growth and Development Assay (LAGDA)

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OECD
OECD
https://www.oecd.org Flag of igo IGO

The Organisation for Economic Co-operation and Development is an intergovernmental organization with 38 member countries, founded in 1961 to stimulate economic progress and world trade. It is a forum the members of which are countries describing themselves as committed to democracy and the market economy, providing a platform to compare policy experiences, seek answers to common problems, identify good practices and coordinate domestic and international policies of its members. The majority of OECD members are high-income economies with a very high Human Development Index (HDI) and are regarded as developed countries.
The test guideline of the Larval Amphibian Growth and Development Assay (LAGDA) describes a toxicity test with an amphibian species (African clawed frog (Xenopus laevis)) that considers growth and development from fertilization through the early juvenile period. It is an assay (typically 16 weeks) that assesses early development, metamorphosis, survival, growth, and partial reproductive maturation. It also enables measurement of a suite of other endpoints that allows for diagnostic evaluation of suspected endocrine disrupting chemicals (EDCs) or other types of developmental and reproductive toxicants. The LAGDA serves as a higher tier test with an amphibian for collecting more comprehensive concentration-response information on adverse effects suitable for use in hazard identification and characterization, and in ecological risk assessment. The general experimental design entails exposing X. laevis embryos at Nieuwkoop and Faber (NF) stage 8-10 (3) to a minimum of four different concentrations of test chem...

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Identifiers

Overton ID
oecddiscovery-6a8f4cef4d47ffbab651405fc693e6f7
doi
10.1787/9789264242340-en

Topics in this document

Gel electrophoresis Analysis of variance ELISA African clawed frog Xenopus Sex reversal Solubility TAE buffer Iodine Toxicity Chemistry Agarose gel electrophoresis Polymerase chain reaction Fixation (histology) Median Water Solvent Biology Statistics Solution (chemistry) Endocrine disruptor Physical sciences Assay Thyroid hormones Aquatic toxicology Chemical substance Estrogen Amphibian Confidence interval Errors and residuals

Related COFOG Divisions

5: Environmental Protection 5.3: Pollution Abatement 7: Health 7.5: R&D Health

Cites research funded by

NERC 1

Citations

Cited by 23 other policy documents (19 of them are from other policy sources)