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oecddiscovery-0d9d59fe49ecbac0c0e586657f2cc0dd
Test No. 203: Fish, Acute Toxicity Test
The fish are exposed to the test substance preferably for a period of 96 hours. Mortalities are recorded at 24, 48, 72 and 96 hours and the concentrations which kill 50 per cent of the fish (LC50) are determined where possible. One or more species may be used, the choice being at the discretion of the testing laboratory. At least seven fishes must be used at each test concentration and in the controls. The test substance should be administered to, at least, five concentrations in a geometric series with a factor preferably not exceeding 2.2. The limit test corresponds to one dose level of 100 mg/L. This study includes the observations of fish at least after 24, 48, 72 and 96 hours. The cumulative percentage mortality for each exposure period is plotted against concentration on logarithmic probability paper.
Topics in this document
Aquatic toxicology
Water
PH
Probit
Total organic carbon
Validity (statistics)
Physical sciences
Chemistry
Toxicity
Chronic toxicity
Solvent
Water quality
Chemical substance
Dissolved organic carbon
Statistics
Solution (chemistry)
Biocide
Alternatives to animal testing
Fathead minnow
Silicone
Solubility
Median lethal dose
OECD
Breathing
Chemical oxygen demand
Purified water
Toxicology
Likelihood function
Citations
Cited by 72
other policy documents
(63 of them are from other policy sources)