Policy document
February 8th 2002

Test No. 420: Acute Oral Toxicity - Fixed Dose Procedure

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OECD
OECD
https://www.oecd.org Flag of igo IGO

The Organisation for Economic Co-operation and Development is an intergovernmental organization with 38 member countries, founded in 1961 to stimulate economic progress and world trade. It is a forum the members of which are countries describing themselves as committed to democracy and the market economy, providing a platform to compare policy experiences, seek answers to common problems, identify good practices and coordinate domestic and international policies of its members. The majority of OECD members are high-income economies with a very high Human Development Index (HDI) and are regarded as developed countries.
A principle of the method is that in the main study only moderately toxic doses are used, and the administration of doses that are expected to be lethal should be avoided. This Guideline is intended primarily for use with rat. Groups of animals of a single sex (normally females) are dosed in a stepwise procedure using the fixed doses of 5, 50, 300 and 2000 mg/kg (exceptionally 5000 mg/kg). The initial dose level is selected on the basis of a sighting study as the dose expected to produce some signs of toxicity without causing severe toxic effects or mortality. Further groups of animals may be dosed at higher or lower fixed doses, depending on the presence or absence of signs of toxicity or mortality. This procedure continues until the dose causing evident toxicity or death is identified, or when no effects are seen at the highest dose or when deaths occur at the lowest dose. The test substance is administered in a single dose by gavage using a stomach tube or a suitable intubation canula. Animals should be...

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Identifiers

Overton ID
oecddiscovery-405b251b4d4b710908883f4288fc66da
doi
10.1787/9789264070943-en

Topics in this document

Dose (biochemistry) Animal testing Clinical medicine Medical specialties Health Globally Harmonized System of Classification and Labelling of Chemicals Median lethal dose Toxicity Medicine Toxicology Occupational safety and health Health sciences Environmental science Physical sciences

Related COFOG Divisions

5: Environmental Protection 5.3: Pollution Abatement 7: Health 7.5: R&D Health

Citations

Cited by 44 other policy documents (43 of them are from other policy sources)