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Identifiers

Overton ID
oecddiscovery-85799dc89554b4c59dcfedbdf0c1c46c
doi
10.1787/9789264203907-en

Test No. 488: Transgenic Rodent Somatic and Germ Cell Gene Mutation Assays

Flag for IGO OECD on June 29th 2020
This Test Guideline describes an in vivo assay that detects chemicals that may induce gene mutations in somatic and germ cells. In this assay, transgenic rats or mice that contain multiple copies of chromosomally integrated plasmid or phage shuttle vectors are used. The transgenes contain reporter genes for the detection of various types of mutations induced by test chemicals. A negative control group and a minimum of 3 treatment groups of transgenic animals are treated for 28 consecutive days. Administration is followed by a period of time, prior to sacrifice, during which the agent is not administered and during which unrepaired DNA lesions are fixed into stable mutations. At the end of this period (usually 3 or 28 days depending on the cell type and requirements), the animals are sacrificed, genomic DNA is isolated from the tissue(s) of interest and purified. Mutations that have arisen during treatment are scored by recovering the transgene and analysing the phenotype of the reporter gene in a bacterial...

Citations

Cited by 46 other policy documents (37 of them are from other policy sources)