Policy document
July 28th 2015

Test No. 493: Performance-Based Test Guideline for Human Recombinant Estrogen Receptor (hrER) In Vitro Assays to Detect Chemicals with ER Binding Affinity

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OECD
OECD
https://www.oecd.org Flag of igo IGO

The Organisation for Economic Co-operation and Development is an intergovernmental organization with 38 member countries, founded in 1961 to stimulate economic progress and world trade. It is a forum the members of which are countries describing themselves as committed to democracy and the market economy, providing a platform to compare policy experiences, seek answers to common problems, identify good practices and coordinate domestic and international policies of its members. The majority of OECD members are high-income economies with a very high Human Development Index (HDI) and are regarded as developed countries.
This Performance-Based Test Guideline (PBTG) describes in vitro assays, which provide the methodology for human recombinant in vitro assays to detect substances with estrogen receptor binding affinity (hrER binding assays). It comprises two mechanistically and functionally similar test methods for the identification of estrogen receptor (i.e. ERα) binders and should facilitate the development of new similar or modified test methods. The two reference test methods that provide the basis for this PBTG are: the Freyberger-Wilson (FW) In Vitro Estrogen Receptor (ER) Binding Assay Using a Full Length Human Recombinant ERα, and the Chemical Evaluation and Research Institute (CERI) In Vitro Estrogen Receptor Binding Assay Using a Human Recombinant Ligand Binding Domain Protein. This assay measures the ability of a radiolabeled ligand ([3H]17β-estradiol) to bind with the ER in the presence of increasing concentrations of a test chemical (i.e. competitor). Test chemicals that possess a high affinity for the ER c...

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Identifiers

Overton ID
oecddiscovery-8f9d855ecc60f651182633a5011e2c65
doi
10.1787/9789264242623-en

Topics in this document

Biochemistry IC50 Hill equation (biochemistry) Regression analysis Hormone Estradiol Ligand binding assay Cell signaling Solubility Experiment Assay Receptor antagonist Endocrine disruptor Estrogen receptor Data analysis Receptor (biochemistry) Ligand (biochemistry) Chemistry Physical sciences Curve fitting Endocrine system Estrogen Molecular binding Reproducibility Estrogen receptor alpha Solution (chemistry) Accuracy and precision G protein-coupled receptor Nuclear receptor Robust statistics

Related COFOG Divisions

7: Health 7.5: R&D Health

Citations

Cited by 26 other policy documents (25 of them are from other policy sources)