Policy document
July 29th 2016

Test No. 487: In Vitro Mammalian Cell Micronucleus Test

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OECD
OECD
https://www.oecd.org Flag of igo IGO

The Organisation for Economic Co-operation and Development is an intergovernmental organization with 38 member countries, founded in 1961 to stimulate economic progress and world trade. It is a forum the members of which are countries describing themselves as committed to democracy and the market economy, providing a platform to compare policy experiences, seek answers to common problems, identify good practices and coordinate domestic and international policies of its members. The majority of OECD members are high-income economies with a very high Human Development Index (HDI) and are regarded as developed countries.
The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells. Micronuclei may originate from acentric chromosome fragments (i.e. lacking a centromere), or whole chromosomes that are unable to migrate to the poles during the anaphase stage of cell division. The assay detects the activity of clastogenic and aneugenic test substances in cells that have undergone cell division during or after exposure to the test substance. This Test Guideline allows the use of protocols with and without the actin polymerisation inhibitor cytochalasin B. Cytochalasin B allows for the identification and selective analysis of micronucleus frequency in cells that have completed one mitosis, because such cells are binucleate. This Test Guideline also allows the use of protocols without cytokinesis block provided there is evidence that the cell population analysed has undergone mitosis.

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Identifiers

Overton ID
oecddiscovery-b4346b746ab8f1b2b966a755ac426793
doi
10.1787/9789264264861-en

Topics in this document

Cytochalasin B Micronucleus test Fluorescence in situ hybridization Cell division Mitosis Micronucleus Genotoxicity Cell biology Biotechnology Biological processes Biology Chromosome Meiosis Telophase Genetics Chemistry Staining Biochemistry Solubility Cell growth Aneuploidy DNA repair Life sciences Experiment Kinetochore Nondisjunction Cytotoxicity Cellular processes

Related COFOG Divisions

5: Environmental Protection 5.3: Pollution Abatement 7: Health 7.5: R&D Health

Citations

Cited by 155 other policy documents (146 of them are from other policy sources)