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Identifiers

Overton ID
oecddiscovery-9f18c73c0eb29180fa45b6258f18bc13
doi
10.1787/9789264076334-en

Test No. 441: Hershberger Bioassay in Rats

Test No. 441: Hershberger Bioassay in Rats

Flag for IGO OECD on September 8th 2009
The Hershberger Bioassay is an in vivo short–term screening test. It evaluates the ability of a chemical to elicit biological activities consistent with androgen agonists, antagonists or 5 á-reductase inhibitors. The current bioassay is based on the changes in weight of five androgen-dependent tissues in the castrate-peripubertal male rat: the ventral prostate, seminal vesicle (plus fluids and coagulating glands), levator ani-bulbocavernosus muscle, paired Cowper’s glands and the glans penis. In order to establish whether a test substance can have androgenic or antiandrogenic action, two – respectively three - dose groups of the test substance, plus positive and vehicle (negative) controls are normally sufficient. The test substance is administered by gavage or subcutaneous injection daily for 10 consecutive days. To test for antiandrogens, the test substance is administered together with a reference androgen agonist. Each treated and control group should include a minimum of 6 animals. The animals ...

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Citations

Cited by 14 other policy documents (11 of them are from other policy sources)