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Overton ID
oecddiscovery-e7092fdac3bc7d8b21a064d60ca662b9
doi
10.1787/9789264122642-en

Test No. 456: H295R Steroidogenesis Assay

Test No. 456: H295R Steroidogenesis Assay

Flag for IGO OECD on July 28th 2011
This Test Guideline describes an in vitro screen for chemical effects on steroidogenesis, specifically the production of 17ß-estradiol (E2) and testosterone (T). The human H295R adreno-carcinoma cell line, used for the assay, expresses genes that encode for all the key enzymes for steroidogenesis. After an acclimation period of 24 h in multi-well plates, cells are exposed for 48 h to seven concentrations of the test chemical in at least triplicate. Solvent and a known inhibitor and inducer of hormone production are run at a fixed concentration as negative and positive controls. At the end of the exposure period, cell viability in each well is analyzed. Concentrations of hormones in the medium can be measured using a variety of methods including commercially available hormone measurement kits and/or instrumental techniques such as liquid chromatography-mass spectrometry. Data are expressed as fold change relative to the solvent control and the Lowest-Observed-Effect-Concentration. If the assay is negative,...

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Citations

Cited by 19 other policy documents (17 of them are from other policy sources)